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Large scale sequencing of transcripts are extensively used for gene discovery and genome annotation. While computational analysis of open reading frames often provides clues to the function of the gene product, it appears to be far more difficult to decode the regulatory information contained in non-coding sequences. The core promoter region immediately upstream of the transcription start site TSS ; plays a crucial role in the assembly of transcription complexes and, as a consequence, in the regulation of transcription initiation. Knowing the precise position of the TSS of a gene thus is of considerable importance for systematic analysis of the regulatory regions of a gene. The oligo-capping cloning technique, which is highly effective in generating cDNA inserts that extend up to the capped 5'end of an mRNA template, efficiently generates data that can be used for large scale and precise TSS mapping. Hundreds of thousands of 5'EST sequences from oligo-capped cDNA libraries mostly from human and Drosophila ; have recently become available. By mapping these sequences to the genome, one can identify the TSS of a large number of genes at once. We introduced the term "in silico primer extention PE ; " to refer to this new approach of promoter mapping. Defining the TSS of genes based on 5' ESTs from oligo-capped cDNA libraries is not as trivial as it might appear. Many genes exhibit a highly dispersed initiation pattern. We have introduced a new clustering method to partition a gene-specific cDNA 5'end profile into one to several promoter regions. For each promoter region, we define the TSS as the most frequently used initiation site. Others have used the longest cDNA sequence of a gene for defining its TSS. However, our approach has a principle advantage to deal with the well known fact that many genes have multiple promoters. We have extensively analyzed the quality of promoter mapping achieved with our specific implementation in silico PE. For those promoters which were already mapped with conventional methods, we observed an almost perfect coincidence of the TSS positions defined by the old and the new method. The assessment of the positional preference and frequency of known promoter elements such as the Initiator or the TATA-box further indicated that in silico PE is at least as precise and accurate as established conventional PE. We used two motif discovery platforms, MEME and Signal Search Analysis, to characterize promoter elements in collections of human and Drosophila promoters defined by in silico PE. Still preliminary analysis of these data sets lead to surprising observations such as the DRE element, functioning in a TRF2-dependent initiation pathway being more frequent than the TATA-box in our Drosophila promoter set. This specific fact may be related to an enrichment of strongly and broadly expressed genes in this promoter collection as compared to previously analyzed data sets. Taken together, these sets might provide evidence for a remarkably higher diversity in core promoter structure than originally thought.
CONTRACT C2311 II. SCOPE OF SERVICE A. General Service Description Purpose Upon request from each institutional Department's Health Services Administrator, the Contractor shall provide all inmate clinical laboratory services as requested by each correctional institution. The Department reserves the option to request specifically designed sensitivity panels and Department profiles as required and as can be performed within the Contractor's capability. B. Rules and Regulations The Contractor shall ensure that all Contractor's staff providing services pursuant to this Contract hereinafter referred to as "Contractor's staff" ; , and the delivery of services under this Contract comply with prevailing ethical and professional standards, Department rules and procedures, including the Department's Health Services' Bulletins Technical Instructions, and memoranda, and all applicable state and federal laws, rules and regulations. C. Medical Records and Documentation The Contractor shall ensure that Contractor's staff document each healthcare encounter in the inmates' medical record, utilizing the SOAP format and specific Department of Corrections' forms. All documentation shall be as outlined in the applicable sections of the Florida Administrative Code, pertinent Department's Health Services' Bulletins Technical Instructions, and applicable Florida Statutes. D. Contractor's Responsibilities 1. The Contractor shall provide specimen pick-up and delivery service to all institutions, on a daily basis, Monday through Friday of each week. Weekend and holiday pick-ups will be offered where there is sufficient demand or need determined by the Department. The Contractor shall establish, with each institution, a schedule of days each week and specific arrival times for specimen pick-up at the respective facility. Adjustments shall be made to these schedules, based upon increased workloads, as mutually agreed upon by the Contractor and the Department. 2. The Contractor shall provide a list of services to each institution that includes the following: a ; overview of services offered by the Contractor; b ; procedures and equipment for collection of specimen for each test; c ; instructions and any special precautions for handling, preserving and packaging specimens for submission to the Contractor's lab; d ; test methodology; and e ; normal values or expected therapeutic ranges. 3. The Contractor shall provide, at no charge to the Department, all supplies necessary solely for the collection, preservation and preparation of specimens submitted to the Contractor for testing and flonase.
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