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The development of intestinal goblet cell hyperplasia hypertrophy during nematode infection involves the Th2 cytokines IL-4 and IL-13 via STAT6 activation. This is thought to play an important role in host protective immunity against the infection. In this study we demonstrate that IL-4 and IL-13 up-regulate the specific goblet cell product trefoil factor-3 TFF3 ; from the mucusproducing HT-29 CL.16E and HT-29 cells selected by adaptation to methotrexate. Up-regulation of TFF3 mRNA and protein levels occurred in a time- and dose-dependent fashion and was accompanied by up-regulation of the goblet cell product mucin 2 MUC2 ; . Addition of actinomycin D before IL-4 IL-13 stimulation led to decreases in TFF3 mRNA levels similar to those observed in controls without IL-4 IL-13. Furthermore, IL-4-mediated increased TFF3 transcription required de novo protein synthesis. Stable transfection of HT-29 CL.16E cells with a truncated dominant-negative form of STAT6 produced a cell line that was unresponsive to IL-4 IL-13. Although only one consensus STAT6 binding site is contained in the TFF3 gene, located in the intron 1, it did not operate as an enhancer in the context of an SV40 promoter luciferase construct. Thus, STAT6 activation mediates a transcriptional enhancement of TFF3 by induction of de novo synthesized protein in goblet cells. The Journal of Immunology, 2004, 172: 37753783. nflammation of the gastrointestinal tract and airways is frequently characterized by CD4 Th cell-mediated mucosal changes that may be either beneficial or detrimental to the epithelial barrier. Th cells differentiate into at least two subsets, Th1 and Th2 cells, based on their cytokine secretion profile 1 ; . Th1 cells, which predominantly produce IL-2, IFN- , and lymphotoxin, are important for the eradication of intracellular pathogens, including bacteria, parasites, yeasts, and viruses. Th2 cells produce IL-4, IL-5, IL-9, and IL-13 and are typical of humoral immune reactions, helminth infections, and atopic diseases such as asthma. Up-regulation of IgE, mastocytosis, eosinophilia, and goblet cell hyperplasia are characteristic of Th2 responses induced by helminth infection and allergic trigger. The production of mucus represents an important response for protecting mucosal surfaces. Paradoxically, Th2 cell-mediated mucus production acts positively in the protection of mucosal surfaces from pathogens 2 ; , but it may be detrimental as well by contributing to airway obstruction during pulmonary inflammatory responses 3 ; . The control of goblet cell production in type 2 inflammatory processes has been attributed to IL-4, IL-9, and IL-13 4, 5 ; . IL-4 and IL-13 are con.
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HUMAN SCABIES: SIGNIFICANTLY HIGHER IGG 4 ANTIBODY RESPONSE TO CRUDE ANTIGENS OF Sarcoptes scabiei var canis AND ITS DIAGNOSTIC POTENTIAL IN DELUSORY CASES OF PARASITOSIS A. Wan Omar1 , S. Naidu1 , A. Hamzah1 , O. Sulaiman2 , K.C. Low2 , S. Yusof2 , N. Rahmah3 and A. A. Khairul4 1 Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Selangor, Malaysia 2 Faculty of Medicine, Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia 3 School of Medical Sciences, Universiti Sains Malaysia, Kelantan, Malaysia 4 Faculty of Medicine, Universiti Malaya, Kuala Lumpur, Malaysia Papulovascular scabies has been misinterpreted as drug eruption or erythema multiforme and nodular scabies as malignant lymphoma. An immunological test may be an alternative method to the diagnosis of delusory parasitosis. To dissect for a predominant immunoglobulin subclass that may has diagnostic potential. Crude mite extract was prepared from mites harvested from rabbits experimentally-infected with Sarcoptes scabiei var canis. Wellcharacterized clinical sera from patients with scabies were evaluated for anti-Sarcoptes antibody classes and subclasses with the aid of ELISA The IgG4 response in patients was significantly higher than the non-infected individuals p 0.01; Students's t-test ; . This imply that IgG4 is the predominant immunoglobulin subclass in scabies infection. IgG4 may serve as a useful serological indicator of diagnostic importance especially in delusory cases of parasitosis. Van Neste DJT. Human scabies in perspective. Int. J. Dermatology 1988; 27 1 ; : 10-14 and propafenone.
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Needs to go on the list, but in terms of giving people a chance to talk in 120 people that's not going to be possible. Speaker 1 ; The reason why people came this evening was to ask the Trust questions about the proposals. Paddy Cooney Which is what we are going to do but we need to give people the opportunity to voice, this forum in this large an area some people find that quite intimidating yet thy want their voice to be heard, so we need to bring the questions together on the table. The questions you have you put on the card, they will come out and they will be answered. Speaker 1 ; So all the questions raised one each table that we have discussed will be asked of the Trust this evening and will be answered this evening. Paddy Cooney Yes. That demands on the table to actually get the discussion, if each one comes up with a hundred questions then we are going to be here till midnight, so but yes the questions are concerning the ones on top of people's minds we need those answers. People need to go away with the answers to those questions yes. Anybody else want any, Peter. New Speaker 2 ; Just a very quick one, its extremely difficult going through those points in the tables if you don't know time scales and I know that when we pressed this at the Overview and Scrutiny Committee last time Roger indicated that he wouldn't be pushed forward on this, but how do you make decisions on in six months time if you don't know what time scales certain things are supposed to be happening. Paddy Cooney We need to come back to time scales at the end and we will do that. This is a way of shaping, I don't want this to be slavishly, you know if you need to discuss other things that have just come out that is fine and I take the issue Peter, I think you are right about some of the time scales that people will need to clarify at the end on where we're going, I understand what you are saying and its going to be slightly imperfect but in terms of trying to use the process to get people engaged we'll need to have some sort of structure. New speaker 3 and quetiapine.
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